重組人R-Spondin-1

重組人R-Spondin-1是一種25.6kDa的蛋白質(zhì)，由243個(gè)氨基酸殘基組成。隨著類器官領(lǐng)域的擴(kuò)大，R-Spondin 1被廣泛用于類器官細(xì)胞培養(yǎng)中，作為促進(jìn)3D類器官生長和存活的重要成分。R-Spondin-1（Rspo-1）屬于Wnt調(diào)節(jié)劑的（Rspo）家族。目前，該家族由四個(gè)結(jié)構(gòu)相關(guān)的分泌配體（Rspo 1-4）組成，所有配體都含有弗林樣結(jié)構(gòu)域和血小板反應(yīng)蛋白結(jié)構(gòu)域。Rspo-1在發(fā)育中的中樞神經(jīng)系統(tǒng)的某些區(qū)域以及腎上腺、卵巢、睪丸、甲狀腺和氣管中表達(dá)。Rspo可以以刺激Wnt/β-catenin信號通路的方式與Frizzled/LRP6受體復(fù)合物相互作用。

(純度) Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).

(內(nèi)毒素) Endotoxin

≤10 EU/mg by the LAL method.

(生物活性) Bioactivity

Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 50 ng/mL wnt3a. The expected ED50 is <1.0 μg/mL.

(種屬) Species

Human

(表達(dá)系統(tǒng)) Expression System

CHO

(預(yù)測分子量大小) Mol. Weight

26.7 kDa

(表達(dá)區(qū)間) Expression Region

Ser21-Ala263

(標(biāo)簽) Tag Info

Tag free

(劑型) Formulation

Lyophilized

(溶液) Buffer

PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.

(規(guī)格) Size

10μg/50μg/1mg

(復(fù)溶) Reconstitution

It is recommended to redissolve in sterile deionized water.

(運(yùn)輸) Shipping

The product is shipped with blue ice.

(存儲(chǔ)) Storage & Stability

36 months at-20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.?

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